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  • Published:

Cell type–specific expression profiling in plants via cell sorting of protoplasts from fluorescent reporter lines

Nature Methods volume 2, pages 615–619 (2005)Cite this article

  • Appropriate growth media

  • 7.5 M ammonium acetate

  • Absolute ethanol

  • Bovine serum albumen (BSA)

  • β-mercaptoethanol (BME)

  • 1 M CaCl2

  • Cellulase (Cellulysin; Calbiochem)

  • GlycoBlue (Ambion)

  • 1 M KCl

  • Mannitol

  • 1 M MgCl2

  • 1 M Tris (unadjusted)

  • 2-(N-Morpholino)-ethanesulfonic acid (MES) hydrate

  • Pectolyase (Sigma)

  • RNeasy Plant Mini Kit (Qiagen)

  • Sterilized seeds

Appropriate growth media

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Figure 1: Overview of protocol for sorting GFP-positive plant protoplasts and applying them to microarrays.
Figure 2: Comparison of negative and positive controls in a two-color scatter plot for cell sorting.

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Authors and Affiliations

  1. Department of Biology, New York University, 100 Washington Square East, New York, 10003, New York, USA

    Kenneth Birnbaum

  2. Department of Biology, Duke University, Box 91000, Durham, 27708, North Carolina, USA

    Jee W Jung, Jean Y Wang & Philip N Benfey

  3. Department of Plant Sciences, University of Arizona, Tucson, 85721, Arizona, USA

    Georgina M Lambert & David W Galbraith

  4. New York University School of Medicine, 401 E. 30th Street, New York, 10016, New York, USA

    John A Hirst

Authors
  1. Kenneth Birnbaum
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  2. Jee W Jung
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  4. Georgina M Lambert
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  7. Philip N Benfey
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Correspondence to Kenneth Birnbaum.

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Birnbaum, K., Jung, J., Wang, J. et al. Cell type–specific expression profiling in plants via cell sorting of protoplasts from fluorescent reporter lines. Nat Methods 2, 615–619 (2005). https://doi.org/10.1038/nmeth0805-615

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