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Software for bead-based registration of selective plane illumination microscopy data

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Figure 1: Bead-based registration framework.


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We thank Carl Zeiss Microimaging for access to the SPIM demonstrator; R.K. Ejsmont (Max Planck Institute of Molecular Cell Biology and Genetics, Dresden) for His-YFP flies; D. White, E. Dimitrova, M. Sarov and P. Campinho for help with imaging and B. Schmid for help with 3D viewer programming. S.P. and S.S. were supported by a Dresden International Graduate School for Biomedicine and Bioengineering doctorate stipend. J.S. and P.T. acknowledge funding from the Human Frontier Science Program Research grant RGY0084.

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Correspondence to Pavel Tomancak.

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Supplementary information

Supplementary Text and Figures

Supplementary Figures 1–8, Supplementary Table 1, Supplementary Methods, Supplementary Data (PDF 4604 kb)

Supplementary Software

SPIM registration plugin for Fiji. (ZIP 4350 kb)

Supplementary Video 1

Rotation capabilities of the selective plane illumination microscope. (MOV 42 kb)

Supplementary Video 2

Visualization of the global optimization of eight view selective plane illumination microscope acquisition of C. elegans with 40×, 0.8 objective. (MOV 4043 kb)

Supplementary Video 3

Visualization of the global optimization of tiled single-photon confocal acquisition of Drosophila with 40×, 0.8 objective. (MOV 6422 kb)

Supplementary Video 4

Three-dimensional rendering of Drosophila gastrulation time-lapse acquired from seven angles. (MOV 393 kb)

Supplementary Video 5

Three-dimensional rendering of Drosophila embryogenesis time-lapse acquired from five angles. (MOV 5121 kb)

Supplementary Video 6

Three-dimensional rendering of fixed Drosophila reconstructed from multiview spinning disc acquisition. (MOV 1108 kb)

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Preibisch, S., Saalfeld, S., Schindelin, J. et al. Software for bead-based registration of selective plane illumination microscopy data. Nat Methods 7, 418–419 (2010).

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