Using mass spectrometry–based proteomics methods, Baerenfaller and colleagues report protein 'catalogs' for cells, seeds and, at different developmental stages, roots, leaves, flowers and fruit (siliques). The researchers found chloroplast and photosynthetic proteins enriched in leaves, intracellular protein transport and oxidative stress response proteins in roots, and heat- and water-sensitive and development-specific proteins in seeds. The identified proteins represent nearly 50% of those predicted from gene models. The data are available in the publicly accessible proteomics identifications database (PRIDE) and can be viewed at their website (http://www.atproteome.ethz.ch/). Happy mining. MP
References
Baerenfaller, K. et al. Genome-scale proteomics reveals Arabidopsis thaliana gene models and proteome dynamics. Science, published online 24 April 2008.
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Pflumm, M. Mapping the Arabidopsis proteome. Nat Methods 5, 463 (2008). https://doi.org/10.1038/nmeth0608-463b
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DOI: https://doi.org/10.1038/nmeth0608-463b