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QuickPALM: 3D real-time photoactivation nanoscopy image processing in ImageJ

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Figure 1: Three-dimensional dSTORM imaging of β-III-tubulin–labeled microtubules in neuronal cells.


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We thank J. Enninga for assistance with experiments, and T. Duong and R. Fesce for helpful insight in the preparation of the manuscript.

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Correspondence to Ricardo Henriques.

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The authors declare no competing financial interests.

Supplementary information

Supplementary Text and Figures

Supplementary Figures 1–2, Supplementary Methods, Supplementary Note 1, Supplementary Discussion (PDF 903 kb)

Supplementary Software 1

QuickPALM software (ZIP 5714 kb)

Supplementary Software 2

Laser control software (ZIP 5596 kb)

Supplementary Movie 1

A 3D presentation of microtubules shown in Figure 1e,f. The viewing angle is rotated here to show different perspectives. Scale bar, 1,000 nm. (MOV 1376 kb)

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Henriques, R., Lelek, M., Fornasiero, E. et al. QuickPALM: 3D real-time photoactivation nanoscopy image processing in ImageJ. Nat Methods 7, 339–340 (2010).

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