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QuickPALM: 3D real-time photoactivation nanoscopy image processing in ImageJ

Nature Methods volume 7, pages 339–340 (2010)Cite this article

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Figure 1: Three-dimensional dSTORM imaging of β-III-tubulin–labeled microtubules in neuronal cells.

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Acknowledgements

We thank J. Enninga for assistance with experiments, and T. Duong and R. Fesce for helpful insight in the preparation of the manuscript.

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Authors and Affiliations

  1. Unidade de Biofísica e Expressão Genética, Instituto de Medicina Molecular, Faculdade de Medicina Universidade de Lisboa, Lisboa, Portugal

    Ricardo Henriques & Musa M Mhlanga

  2. Institut Pasteur, Groupe Imagerie et Modélisation, Institut Pasteur, Groupe Imagerie et Modélisation; Centre National de la Recherche Scientifique,

    Ricardo Henriques, Mickael Lelek & Christophe Zimmer

  3. Centre National de la Recherche Scientifique, Unité de Recherche Associée 2582, Paris, France

    Ricardo Henriques, Mickael Lelek & Christophe Zimmer

  4. Istituto Scientifico San Raffaele, Università Vita-Salute, Milano, Italia

    Eugenio F Fornasiero & Flavia Valtorta

  5. Unità di Neuroscienze Molecolari, Istituto Italiano di Tecnologia, Milano, Italia

    Eugenio F Fornasiero & Flavia Valtorta

  6. Gene Expression and Biophysics Group, Synthetic Biology Emerging Research Area, Council for Scientific and Industrial Research, Pretoria, South Africa

    Musa M Mhlanga

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  1. Ricardo Henriques
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Correspondence to Ricardo Henriques.

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Supplementary information

Supplementary Text and Figures

Supplementary Figures 1–2, Supplementary Methods, Supplementary Note 1, Supplementary Discussion (PDF 903 kb)

Supplementary Software 1

QuickPALM software (ZIP 5714 kb)

Supplementary Software 2

Laser control software (ZIP 5596 kb)

Supplementary Movie 1

A 3D presentation of microtubules shown in Figure 1e,f. The viewing angle is rotated here to show different perspectives. Scale bar, 1,000 nm. (MOV 1376 kb)

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Henriques, R., Lelek, M., Fornasiero, E. et al. QuickPALM: 3D real-time photoactivation nanoscopy image processing in ImageJ. Nat Methods 7, 339–340 (2010). https://doi.org/10.1038/nmeth0510-339

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