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Super-resolution light microscopy goes live

Nature Methods volume 5, pages 385–387 (2008)Cite this article

Microscopic resolution far beyond the diffraction limit is possible by localizing single molecules individually. This approach has now been demonstrated on living cells.

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Figure 1: The concept of live, time series PALM.

References

  1. Shroff, H., Galbraith, C.G., Galbraith, J.A. & Betzig, E. Nat. Methods 5, 417–423 (2008).

    Article  CAS  Google Scholar 

  2. Gustafsson, M.G.L. et al. Biophys. J., published online 7 March 2008 (doi:10.1529/biophysj.107.120345).

    Article  CAS  Google Scholar 

  3. Donnert, G. et al. Proc. Natl. Acad. Sci. USA 103, 11440–11445 (2006).

    Article  CAS  Google Scholar 

  4. Schwentker, M.A. et al. Microsc. Res. Tech. 70, 269–280 (2007).

    Article  CAS  Google Scholar 

  5. Gustafsson, M.G.L. Proc. Natl. Acad. Sci. USA 102, 13081 (2005).

    Article  CAS  Google Scholar 

  6. Betzig, E. et al. Science 313, 1642–1645 (2006).

    Article  CAS  Google Scholar 

  7. Hess, S.T. Girirajan, T.P.K. & Mason, M.D. Biophys. J. 91, 4258–4272 (2006).

    Article  CAS  Google Scholar 

  8. Rust, M.J., Bates, M. & Zhuang, X. Nat. Methods 3, 793–795 (2006).

    Article  CAS  Google Scholar 

  9. Westphal, V. et al. Science, published online 21 February 2008 (10.1126/science.1154228).

  10. Manley, S. et al. Nat. Methods 5, 155–157 (2008).

    Article  CAS  Google Scholar 

  11. Hess, S.T. et al. Proc. Natl. Acad. Sci. USA 104, 17379–17375 (2007).

    Article  Google Scholar 

Download references

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Authors and Affiliations

  1. Mats G. L. Gustafsson is in the Department of Physiology and Program in Bioengineering at the University of California San Francisco, Box 2532, 1700 4th Street, San Francisco, California 94143, USA. mats@msg.ucsf.edu,

    Mats G L Gustafsson

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  1. Mats G L Gustafsson
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Gustafsson, M. Super-resolution light microscopy goes live. Nat Methods 5, 385–387 (2008). https://doi.org/10.1038/nmeth0508-385

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