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Splitting or stacking fluorescent proteins to visualize mRNA in living cells

Several new approaches to tag mRNA allow real-time imaging of mRNA dynamics in living bacteria, yeast and human cells.

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Figure 1: Imaging endogenous mRNA in yeast.

Kim Caesar

Figure 2: Split GFP complementation for tagging mRNA in live cells.

Kim Caesar

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Acknowledgements

The author's research is supported by US National Institutes of Health grant GM-070357.

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Tyagi, S. Splitting or stacking fluorescent proteins to visualize mRNA in living cells. Nat Methods 4, 391–392 (2007). https://doi.org/10.1038/nmeth0507-391

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