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Photo-cross-linking interacting proteins with a genetically encoded benzophenone

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Figure 1: Structure of pBpa and a schematic of the pDULE plasmid.
Figure 2: The steps to express a protein with a site-specifically incorporated pBpa in E. coli.
Figure 3: Analysis of pBpa-containing protein production.
Figure 4: Irradiation time courses of HDH-225pBpa and HDH-225Tyr.


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J.W.C. and R.A.M. thank Peter Schultz for his support in the development of some of the vectors described in this protocol.

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Correspondence to Ryan A Mehl or Jason W Chin.

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Farrell, I., Toroney, R., Hazen, J. et al. Photo-cross-linking interacting proteins with a genetically encoded benzophenone. Nat Methods 2, 377–384 (2005).

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