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Lifeact mice for studying F-actin dynamics

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Figure 1: Characterization of transgenic Lifeact mice.

References

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Acknowledgements

This work was supported by the Max Planck society.

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Correspondence to Michael Sixt or Roland Wedlich-Söldner.

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Competing interests

J.R., M.S. and R.W.-S. have submitted a patent application (07 023 084.2; Peptide for determining actin structures in living cells) to the EU patent office.

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Supplementary Text and Figures

Supplementary Figures 1–6, Supplementary Methods (PDF 1847 kb)

Supplementary Video 1

Migrating T-cell expressing Lifeact-EGFP. Images were acquired by TIRFM. Scale bar, 3 μm. (MOV 308 kb)

Supplementary Video 2

Spreading platelet expressing Lifeact-EGFP. Images were acquired by TIRFM. Scale bar, 3 μm. (MOV 58 kb)

Supplementary Video 3

Dividing chondrocytes. Images of tibial cartilage slice cultures were acquired on a spinning disk microscope. Scale bar, 20 μm. (MOV 364 kb)

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Riedl, J., Flynn, K., Raducanu, A. et al. Lifeact mice for studying F-actin dynamics. Nat Methods 7, 168–169 (2010). https://doi.org/10.1038/nmeth0310-168

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  • DOI: https://doi.org/10.1038/nmeth0310-168

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