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We are grateful to S. Kondo and A. Hasegawa for help with bioinformatics, and H. Sato, C. Kawazu, S. Kanagawa, M. Ohno, M. Murata, K. Nomura, Y. Tagami-Takeda and K. Hayashida for support in developing, producing and sequencing CAGE libraries. This protocol was developed with the support of the Genome Network Project, the Advanced and Innovational Research Program in Life Science and the Research Grant for RIKEN Genome Exploration Research Project, all from the Ministry of Education, Culture, Sports, Science and Technology, as well as the Strategic Programs for Research and Development of RIKEN. R.K. was supported by a fellowship from the European Union (FP5 INCO2 to Japan).
Flowchart for the high-throughput preparation of full-length enriched cDNAs. (PDF 149 kb)
Oligonucleotides for use in CAGE protocol. (DOC 26 kb)
CAGE Linker Oligos. (PDF 312 kb)
HPLC gradient used for fractionation of concatemers. (DOC 563 kb)
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Kodzius, R., Kojima, M., Nishiyori, H. et al. CAGE: cap analysis of gene expression. Nat Methods 3, 211–222 (2006). https://doi.org/10.1038/nmeth0306-211
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