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Profiling DNA methylation patterns using genomic tiling microarrays

Nature Methods volume 2, pages 219–224 (2005)Cite this article

  • BSA (10 mg/ml)

  • DNA sample to be assayed

  • dNTP solution (10 mM each)

  • 100 mM GTP

  • McrBC enzyme and buffer (New England BioLabs)

  • 10× Mg2+ buffer: 500 mM Tris-HCl (pH 8.0), 150 mM MgCl2

  • 25 mM MgCl2

  • 5 M NaCl

  • Orange G loading dye (6×)

  • Primer A (40 pmol/μl): 5′-GTTTCCCAGTCACGATCNNNNNN-3′

  • Primer B (100 pmol/μl): 5′-GTTTCCCAGTCACGATC-3′

  • QIAquick Gel Extraction and PCR Purification kits (Qiagen)

  • Sequenase (13 U/μl; US Biochemicals)

  • Taq DNA polymerase (Roche Applied Science)

BSA (10 mg/ml)

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Figure 1: Scheme for DNA methylation profiling.

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Authors and Affiliations

  1. Watson School of Biological Sciences and Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, 11724, New York, USA

    Zachary Lippman & Rob Martienssen

  2. Unité de Recherche en Génomique Végétale (URGV), INRA/CNRS/UEVE, Rue Gaston Crémieux, Evry, 91057, Cedex, France

    Anne-Valérie Gendrel & Vincent Colot

Authors
  1. Zachary Lippman
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  2. Anne-Valérie Gendrel
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  3. Vincent Colot
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  4. Rob Martienssen
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Corresponding authors

Correspondence to Vincent Colot or Rob Martienssen.

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Lippman, Z., Gendrel, AV., Colot, V. et al. Profiling DNA methylation patterns using genomic tiling microarrays. Nat Methods 2, 219–224 (2005). https://doi.org/10.1038/nmeth0305-219

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