Skip to main content

Thank you for visiting You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Mind the gaps

By grouping short reads derived from the same long genomic fragment, the reads can easily be assembled into fragments that approach the length of capillary sequencing reads.

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Get just this article for as long as you need it


Prices may be subject to local taxes which are calculated during checkout

Figure 1: Turning short reads into long reads using subassembly.


  1. Hiatt, J.B., Patwardhan, R.P., Turner, E.H., Lee, C. & Shendure, J. Nat. Methods 7, 119–122 (2010).

    Article  CAS  Google Scholar 

  2. Li, R. et al. Nature advance online publication, doi:10.1038/nature08696 (13 December 2009).

  3. DeLong, E.F. Nat. Rev. Microbiol. 3, 459–469 (2005).

    Article  CAS  Google Scholar 

  4. Turnbaugh, P.J. et al. Nature 449, 804–810 (2007).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations


Ethics declarations

Competing interests

The author declares no competing financial interests.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Salzberg, S. Mind the gaps. Nat Methods 7, 105–106 (2010).

Download citation

  • Issue Date:

  • DOI:


Quick links

Nature Briefing: Translational Research

Sign up for the Nature Briefing: Translational Research newsletter — top stories in biotechnology, drug discovery and pharma.

Get what matters in translational research, free to your inbox weekly. Sign up for Nature Briefing: Translational Research