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The HA tag is cleaved and loses immunoreactivity during apoptosis

Nature Methods volume 4, pages 107108 (2007) | Download Citation

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Author information

Affiliations

  1. INSERM E347, Institut Bergonié, 229 Cours de l'Argonne, 33076 Bordeaux, France.

    • Laura Schembri
    • , Renee Dalibart
    • , Flora Tomasello
    • , François Ichas
    •  & Francesca De Giorgi
  2. CNRS UMR5164, Université Bordeaux 2, 146 rue Léo Saignat, 33076 Bordeaux, France.

    • Patrick Legembre

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Competing interests

L.S., F.T., F.I. and F.D.G. are authors on a patent application WO, 2005/012913.

Corresponding author

Correspondence to Francesca De Giorgi.

Supplementary information

PDF files

  1. 1.

    Supplementary Figure 1

    SNAP-HAGFP signal redistribution during apoptosis.

  2. 2.

    Supplementary Figure 2

    SNAP-HAGFP cleavage occurs early during apoptosis.

  3. 3.

    Supplementary Figure 3

    Active caspase 3 cleaves SNAP-HAGFP.

  4. 4.

    Supplementary Figure 4

    SNAP-mutHAGFP (DVPD of HA replaced by DVPE) is not cleavable and does not react with the anti-HA antibody.

  5. 5.

    Supplementary Figure 5

    HA cleavage occurs irrespective of the nature and the location of the construct.

Videos

  1. 1.

    Supplementary Video 1

    Live cell imaging of HA cleavage.

About this article

Publication history

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DOI

https://doi.org/10.1038/nmeth0207-107

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