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The HA tag is cleaved and loses immunoreactivity during apoptosis

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Figure 1: The HA tag is cleaved by caspase 3/7.

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Correspondence to Francesca De Giorgi.

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Competing interests

L.S., F.T., F.I. and F.D.G. are authors on a patent application WO, 2005/012913.

Supplementary information

Supplementary Figure 1

SNAP-HAGFP signal redistribution during apoptosis.

Supplementary Figure 2

SNAP-HAGFP cleavage occurs early during apoptosis.

Supplementary Figure 3

Active caspase 3 cleaves SNAP-HAGFP.

Supplementary Figure 4

SNAP-mutHAGFP (DVPD of HA replaced by DVPE) is not cleavable and does not react with the anti-HA antibody.

Supplementary Figure 5

HA cleavage occurs irrespective of the nature and the location of the construct.

Supplementary Video 1

Live cell imaging of HA cleavage.

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Schembri, L., Dalibart, R., Tomasello, F. et al. The HA tag is cleaved and loses immunoreactivity during apoptosis. Nat Methods 4, 107–108 (2007). https://doi.org/10.1038/nmeth0207-107

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  • DOI: https://doi.org/10.1038/nmeth0207-107

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