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The HA tag is cleaved and loses immunoreactivity during apoptosis

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Figure 1: The HA tag is cleaved by caspase 3/7.


  1. Field, J. et al. Mol. Cell. Biol. 8, 2159–2165 (1988).

    CAS  Article  Google Scholar 

  2. De Giorgi, F. et al. Methods Cell. Biol. 58, 75–85 (1999).

    CAS  Article  Google Scholar 

  3. Pochampally, R. et al. Oncogene 17, 2629–2636 (1998).

    CAS  Article  Google Scholar 

  4. Katsuda, K. et al. Oncogene 21, 2108–2113 (2002).

    CAS  Article  Google Scholar 

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Correspondence to Francesca De Giorgi.

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Competing interests

L.S., F.T., F.I. and F.D.G. are authors on a patent application WO, 2005/012913.

Supplementary information

Supplementary Figure 1

SNAP-HAGFP signal redistribution during apoptosis.

Supplementary Figure 2

SNAP-HAGFP cleavage occurs early during apoptosis.

Supplementary Figure 3

Active caspase 3 cleaves SNAP-HAGFP.

Supplementary Figure 4

SNAP-mutHAGFP (DVPD of HA replaced by DVPE) is not cleavable and does not react with the anti-HA antibody.

Supplementary Figure 5

HA cleavage occurs irrespective of the nature and the location of the construct.

Supplementary Video 1

Live cell imaging of HA cleavage.

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Schembri, L., Dalibart, R., Tomasello, F. et al. The HA tag is cleaved and loses immunoreactivity during apoptosis. Nat Methods 4, 107–108 (2007).

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