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Single-molecule DNA nanomanipulation: Improved resolution through use of shorter DNA fragments

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Figure 1: Experimental approach.
Figure 2: Preparation of DNA fragment constructs.
Figure 3: Detection of RNAP-dependent promoter unwinding: improved resolution through use of shorter DNA fragment.

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Acknowledgements

We thank S. Adhya for plasmid samples. This work was supported by funds from the Institut Jacques Monod, an Action Thématique et Incitative sur Programme grant from the Centre National de la Recherche Scientifique, the Universities of Paris VI and Paris VII, the Fondation pour la Recherche Médicale, and a Cold Spring Harbor Laboratory Fellowship to T.R.S., and by National Institutes of Health grant GM41376 and a Howard Hughes Medical Institute Investigatorship to R.H.E.

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Correspondence to Richard H Ebright or Terence R Strick.

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Revyakin, A., Ebright, R. & Strick, T. Single-molecule DNA nanomanipulation: Improved resolution through use of shorter DNA fragments. Nat Methods 2, 127–138 (2005). https://doi.org/10.1038/nmeth0205-127

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  • DOI: https://doi.org/10.1038/nmeth0205-127

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