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Reduce, reuse, reprogram

Nature Methods volume 7, pages 39–40 (2010)Cite this article

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Mouse lines with inducible reprogramming factors expressed from a single genomic locus will allow reprogramming studies in multiple cell types and defined genetic backgrounds.

Epigenetic reprogramming is a process in which the ectopic overexpression of defined transcription factors (typically Oct4, Sox2, Klf4 and c-Myc) in differentiated somatic cells initiates a complex and poorly understood process by which the cells regress developmentally into a state similar to that of embryonic stem cells (ESCs). This approach, which generates what are termed 'induced pluripotent stem' cells (iPSCs), has attracted considerable interest from the entire biomedical research community and has triggered many studies ranging from basic molecular and cellular research to efforts aimed at the generation of clinically relevant cells. Two papers published in this issue of Nature Methods, from groups led by Rudolf Jaenisch and by Konrad Hochedlinger, describe a system for making iPSCs that has the potential to drastically accelerate current efforts toward an understanding of the reprogramming process1,2.

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Figure 1: Two inducible iPSC systems.

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  1. Thomas P. Zwaka is at Baylor College of Medicine, Houston, Texas, USA.,

    Thomas P Zwaka

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  1. Thomas P Zwaka
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Correspondence to Thomas P Zwaka.

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Zwaka, T. Reduce, reuse, reprogram. Nat Methods 7, 39–40 (2010). https://doi.org/10.1038/nmeth0110-39

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  • DOI: https://doi.org/10.1038/nmeth0110-39

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