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BRET analysis of GPCR oligomerization: newer does not mean better

Nature Methods volume 4, pages 3–4 (2007)Cite this article

To the editor: James et al.1 proposed a “rigorous” treatment of bioluminescence resonance energy transfer (BRET) data to distinguish random (nonspecific) from true oligomeric protein interactions. The question is not trivial, and the intention is laudable as BRET is becoming increasingly popular with more than 100 original articles published using the method. James et al., however, dismissed many studies that addressed similar issues, and several points deserve comments.

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References

  1. James, J.R., Oliveira, M.I., Carmo, A.M., Iaboni, A. & Davis, S.J. Nat. Methods 3, 1001–1006 (2006).

    CAS  Article  Google Scholar 

  2. Mercier, J.F., Salahpour, A., Angers, S., Breit, A. & Bouvier, M. J. Biol. Chem 277, 44925–44931 (2002).

    CAS  Article  Google Scholar 

  3. Issafras, H. et al. J. Biol. Chem. 277, 34666–34673 (2002).

    CAS  Article  Google Scholar 

  4. Percherancier, Y. et al. J. Biol. Chem. 280, 9895–9903 (2005).

    CAS  Article  Google Scholar 

  5. Ramsay, D., Kellett, E., McVey, M., Rees, S. & Milligan, G. Biochem. J. 365, 429–440 (2002).

    CAS  Article  Google Scholar 

  6. Urizar, E. et al. EMBO J. 24, 1954–1964 (2005).

    CAS  Article  Google Scholar 

  7. Ayoub, M.A. et al. J. Biol. Chem. 277, 21522–21528 (2002).

    CAS  Article  Google Scholar 

  8. Hanyaloglu, A.C., Seeber, R.M., Kohout, T.A., Lefkowitz, R.J. & Eidne, K.A. J. Biol. Chem. 277, 50422–50430 (2002).

    CAS  Article  Google Scholar 

  9. Kenworthy, A.K. & Edidin, M. J. Cell. Biol. 142, 69–84 (1998).

    CAS  Article  Google Scholar 

  10. Terrillon, S. et al. Mol. Endocrinol. 17, 677–691 (2003).

    CAS  Article  Google Scholar 

  11. Breit, A., Lagacé, M. & Bouvier, M. J. Biol. Chem. 279, 28756–28765 (2004).

  12. Anger, S. et al. Proc. Natl. Acad. Sci. USA 97, 3684–3689 (2000).

    Google Scholar 

  13. Wilson, S., Wilkinson, G. & Milligan, G. J.Biol. Chem. 280, 28663–28674 (2005).

    CAS  Article  Google Scholar 

  14. Levoye, A. et al. EMBO J. 25, 3012–3023 (2006).

    CAS  Article  Google Scholar 

  15. Ayoub, M.A., Levoye, A., Delagrange, P. & Jockers, R. Mol. Pharmacol. 66, 312–321 (2004).

    CAS  Article  Google Scholar 

  16. Milligan, G. & Bouvier, M. FEBS J. 272, 2914–2925 (2005).

    CAS  Article  Google Scholar 

  17. Bulenger, S., Marullo, S. & Bouvier, M. Trends Pharmacol. Sci. 26, 131–137 (2005).

    CAS  Article  Google Scholar 

Download references

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Authors and Affiliations

  1. Institute of Research in Immunology and Cancer, Department of Biochemistry, Université de Montréal, C.P. 6128 Succursale Centre-Ville, Montréal, Québec, H3C 3J7, Canada

    Michel Bouvier

  2. Ste-Justine Hospital Research Center, Department of Biochemistry, Université de Montréal, 3175 Chemin de la Côte Sainte-Catherine, Montréal, Québec, H3T 1C5, Canada

    Nikolaus Heveker

  3. Institut Cochin, Département de Biologie Cellulaire, INSERM, U567,

    Ralf Jockers & Stefano Marullo

  4. Centre National de la Recherche Scientifique, Unité Mixte de Recherche 8104,

    Ralf Jockers & Stefano Marullo

  5. Université Paris 5, Faculté de Médecine René Descartes, UM 3, Paris, F-75014, France

    Ralf Jockers & Stefano Marullo

  6. Division of Biochemistry and Molecular Biology, Institute of Biomedical & Life Sciences, University of Glasgow, Wolfson Building, Glasgow, G12 8QQ, UK

    Graeme Milligan

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  1. Michel Bouvier
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Bouvier, M., Heveker, N., Jockers, R. et al. BRET analysis of GPCR oligomerization: newer does not mean better. Nat Methods 4, 3–4 (2007). https://doi.org/10.1038/nmeth0107-3

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  • DOI: https://doi.org/10.1038/nmeth0107-3

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