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Transfection of mammalian cells by electroporation

Pulsed electrical fields can be used to introduce DNA into a wide variety of animal cells1,2. Electroporation works well with cell lines that are refractive to other techniques, such as calcium phosphate–DNA coprecipitation. But as with other transfection methods, the optimal conditions for electroporation of untested cell lines must be determined experimentally.

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References

  1. Wong, T.-K. & Neumann, E. Electric field mediated gene transfer. Biochem. Biophys. Res. Commun. 107, 584–587 (1982).

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  2. Potter, H., Weir, L. & Leder, P. Enhancer-dependent expression of human κ immunoglobulin genes introduced into mouse pre-B lymphocytes by electroporation. Proc. Natl. Acad. Sci. USA 81, 7161–7165 (1984).

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Transfection of mammalian cells by electroporation. Nat Methods 3, 67–68 (2006). https://doi.org/10.1038/nmeth0106-67

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  • DOI: https://doi.org/10.1038/nmeth0106-67

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