The figure illustrates an example of five-channel acquisition with illumination, filter wheel, and Z motor control via hardware triggering. The camera exposures are observed by the NIDAQ. NIDAQ output signaling controls the excitation wavelengths and timing such that they occur in subsequent frames of the time lapse as transmitted (Tx) light or UV, blue, green, or red excitation wavelengths. The emission (Em) filter wheel position is also shown after the exposure, and because this device is slower, the movement duration is indicated in red. Note that the movement is synchronized with times when the detector is not actively exposing. Finally, the piezo Z motor position follows the exposure signal and also the frame number, so the movement occurs only after one frame loop is complete.