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Acknowledgements

We thank R. Bittman of the City University of New York for providing Bodipy488-cholesterol, and the members of the Kusumi laboratory for helpful discussions.

Author information

Author notes

    • Kenji A K Tanaka
    •  & Kenichi G N Suzuki

    These authors contributed equally to this work.

Affiliations

  1. Membrane Mechanisms Project, International Cooperative Research Project, Japan Science and Technology Agency (JST), Institute for Integrated Cell-Material Sciences (iCeMS) and Research Center for Nano Medical Engineering, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan.

    • Kenji A K Tanaka
    • , Yuki M Shirai
    • , Shusaku T Shibutani
    • , Manami S H Miyahara
    • , Hisae Tsuboi
    • , Miyako Yahara
    •  & Akihiro Kusumi
  2. Precursory Research for Embryonic Science and Technology, JST, iCeMS, Kyoto University, Kyoto, Japan.

    • Kenichi G N Suzuki
  3. Department of Microbiology and Immunology, School of Medicine, Keio University and Core Research for Evolutional Science and Technology, JST, Tokyo, Japan.

    • Akihiko Yoshimura
  4. National Centre for Biological Sciences, Bangalore, India.

    • Satyajit Mayor
  5. Center for Meso-Bio Single-Molecule Imaging, iCeMS, Kyoto University, Kyoto, Japan.

    • Takahiro K Fujiwara
    •  & Akihiro Kusumi

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Competing interests

The authors declare no competing financial interests.

Corresponding author

Correspondence to Akihiro Kusumi.

Supplementary information

PDF files

  1. 1.

    Supplementary Text and Figures

    Supplementary Figures 1–6, Supplementary Methods

Videos

  1. 1.

    Supplementary Video 1

    A typical video clip showing the diffusion of single Halo-GPI molecules on the apical plasma membrane at 37 °C. Before chemical cross-linking (untreated, left), and after treatment with 4% PFA for 30 min (center) or with 4% PFA + 0.1% GA for 30 min (right).

  2. 2.

    Supplementary Video 2

    A typical video clip showing the diffusion of single Halo–H-Ras molecules on the apical plasma membrane at 37 °C. Before chemical cross-linking (untreated, left), and after treatment with 4% PFA for 30 min (center) or with 4% PFA + 0.1% GA for 30 min (right).

About this article

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DOI

https://doi.org/10.1038/nmeth.f.314

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