Figure 2 : Enrichment of A-B–tagged DNA fragments.

From: Next-generation sequencing library preparation: simultaneous fragmentation and tagging using in vitro transposition

Figure 2

Transposon ends were modified to contain two unique Roche/454–compatible tags (A and B), and a library was prepared following the scheme in Figure 1b. qPCR was performed using A-A, B-B and A-B primer pairs for 45 cycles. The horizontal orange line is the fluorescence threshold.