Grant, T. et al. eLife 7, e35383 (2018).

The rapidly growing interest in cryo-electron microscopy (cryo-EM), driven by major technological advances in recent years, has increased the demand for powerful yet user-friendly software tools. Grant et al. present cisTEM (computational imaging system for transmission electron microscopy), a complete software solution for single-particle cryo-EM data analysis, from raw image processing to structure determination. cisTEM was built from existing and novel algorithms that implement essential steps such as movie processing, image defocus determination, particle picking, 2D classification, 3D map generation, and 3D refinement. This open-source software program is designed to run on high-end CPU workstations and contains an easy-to-use graphical user interface. Grant et al. demonstrate the performance of cisTEM, using b-galactosidase as an example.