Correspondence | Published:

Computational correction of index switching in multiplexed sequencing libraries

Nature Methods volume 15, pages 305307 (2018) | Download Citation

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1.

    et al. Cell Metab. 24, 593–607 (2016).

  2. 2.

    et al. Science 357, 661–667 (2017).

  3. 3.

    Illumina Inc. Effects of Index Misassignment on Multiplexing and Downstream Analysis. Publication No. 770-2017-004-C QB # 5420 (Illumina Inc., 2017).

  4. 4.

    et al. bioRxiv preprint at (2017).

  5. 5.

    , , , & bioRxiv preprint at (2018).

  6. 6.

    et al. bioRxiv preprint at (2017).

Download references

Acknowledgements

This work was supported by the Swedish Research Council (grant 2017-01062 to R. Sandberg), the Bert L. and N. Kuggie Vallee Foundation (to R. Sandberg), a National Science Foundation Graduate Research Fellowship (grant DGE 1147470 to G.S.), the NIH (grant R01CA86065 to I.L.W.), the California Institute for Regenerative Medicine (grant GC1R-06673-A), to M. Snyder; Collaborative Reserch Project to I.L.W. and the Virginia and D.K. Ludwig Fund for Cancer Research (to I.L.W.)

Author information

Affiliations

  1. Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.

    • Anton J M Larsson
    •  & Rickard Sandberg
  2. Department of Bioengineering, Stanford University School of Engineering, Stanford, California, USA.

    • Geoff Stanley
  3. Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, California, USA.

    • Rahul Sinha
    •  & Irving L Weissman

Authors

  1. Search for Anton J M Larsson in:

  2. Search for Geoff Stanley in:

  3. Search for Rahul Sinha in:

  4. Search for Irving L Weissman in:

  5. Search for Rickard Sandberg in:

Competing interests

The authors declare no competing financial interests.

Corresponding author

Correspondence to Rickard Sandberg.

Integrated supplementary information

Supplementary information

PDF files

  1. 1.

    Supplementary Text and Figures

    Supplementary Figures 1–4 and Supplementary Methods

  2. 2.

    Life Sciences Reporting Summary

Zip files

  1. 1.

    Supplementary Software

    unspread software for index switching correction

About this article

Publication history

Published

DOI

https://doi.org/10.1038/nmeth.4666

Newsletter Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing