(a) Self-organizing map (SOM) of z-score-transformed pseudo-temporal expression profiles along the pDC developmental trajectory derived from the t-SNE map in Fig. 3a. Example profiles are shown for four genes dynamically expressed during pDC differentiation. The black line indicates a local regression. The SOM has been computed for 711 cells with predicted pDC fate bias >0.15 derived from three independent experiments with n = 4 mice. (b) Shown are t-SNE maps highlighting normalized transcript levels of Il7r, Cd34, Csf1r and Ly6d for 1,802 cells from three independent experiments with n = 4 mice.