(a-e) The heatmaps show genes with a random forests importance measure >0.02 and a ratio between the absolute importance and its standard deviation >2 for at least a single iteration. Iterations are depicted on the x-axis with the first iteration to the left and the final iteration to the right. Early iterations correspond to more mature stages while late iterations correspond to more naïve stages. A hierarchical clustering dendogram is indicated on the left margin. Heatmaps are shown for (a) the neutrophil, (b) the pDC, (c) the erythrocyte, (d) the cDC, and (e) the NK/NKT/ILC lineage. (f) Heatmap of Spearman’s correlation coefficient between the fate bias predicted by FateID and cell surface marker expression. An elastic-net regularized linear regression by the normal family as used by Velten et al.1 confirms the same trends, but the correlation-based analysis better discriminates the sub-population corresponding to distinct lineages. In (a-f) data derived from 1,802 cells from three independent experiments with n = 4 mice are shown.
1. Velten, L. et al. Human haematopoietic stem cell lineage commitment is a continuous process. Nat. Cell Biol. 19, 271–281 (2017).