(A) Spatial expression patterns of significant genes (by at least one of the four statistical tests, Benjamini-Hochberg adjusted P < 0.05, two-sided) for spatial-transcriptomics breast-cancer dataset (layer 2, n=251 array-spots). Expression levels were scaled to the range [0-1] for visualisation purposes. Red circles in the density plots (B) indicate cells in regions with significantly higher expression (one-sided kernel density estimation, P ≤ 0.05). (C) Heatmap with genes by row and cells by column, where black indicate that a cell was deemed by trendsceek to be in a region of significantly upregulated expression. Upper bar indicates cell-clusters and left bar gene-clusters (agglomerative hierarchical clustering, Euclidean distance, Ward’s criterion). (D) Spatial layout of the cell- and gene-clusters identified in (C).