Supplementary Figure 4: Full-length (FL) immunogens provide efficiencies comparable to those of domain antigens. | Nature Methods

Supplementary Figure 4: Full-length (FL) immunogens provide efficiencies comparable to those of domain antigens.

From: A toolbox of immunoprecipitation-grade monoclonal antibodies to human transcription factors

Supplementary Figure 4

Key to the graphs represent abbreviations for mAb grouping using a single immunogen for immunization (see online methods for details). D-A-f.p. =Domain-All-footpad. F-F-f.p. = Full length-Full length- footpad (a) z-/S- score are higher in D-A-f.p. (n=174) versus the F-F-f.p (n=530) mAbs. Mean ± S.D.DAfp = 58.36 ± 79.18, nDFA=174; Mean ± S.DFFfp= 89.06 ± 32.05, nDDA=530. * and *** represents p=0.01 and 1.39E-06 respectively by Benjamini-Hochberg FDR (b) Comparing success rates of D-A-f.p. (n=33) and F-F-f.p (n=215) mAbs at different stages of the validation pipeline.

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