Subscribe to Journal
Get full journal access for 1 year
only $4.92 per issue
All prices are NET prices.
VAT will be added later in the checkout.
Tax calculation will be finalised during checkout.
Rent or Buy article
Get time limited or full article access on ReadCube.
All prices are NET prices.
Schaefer, K.A. et al. Nat. Methods 14, 547–548 (2017).
Saunders, C.T. et al. Bioinformatics 28, 1811–1817 (2012).
Cibulskis, K. et al. Nat. Biotechnol. 31, 213–219 (2013).
Rimmer, A. et al. Nat. Genet. 46, 912–918 (2014).
Kim, D. et al. Nat. Methods 12, 237–243 (2015).
Cho, S.W., Kim, S., Kim, J.M. & Kim, J.-S. Nat. Biotechnol. 31, 230–232 (2013).
Cho, S.W. et al. Genome Res. 24, 132–141 (2014).
Smith, C. et al. Cell Stem Cell 15, 12–13 (2014).
Veres, A. et al. Cell Stem Cell 15, 27–30 (2014).
Suzuki, K. et al. Cell Stem Cell 15, 31–36 (2014).
Iyer, V. et al. Nat. Methods 12, 479 (2015).
Tsai, S.Q. et al. Nat. Biotechnol. 33, 187–197 (2015).
Frock, R.L. et al. Nat. Biotechnol. 33, 179–186 (2015).
J.-S.K. is supported by a grant from the Institute for Basic Science (IBS-R021-D1).
J.-S.K. is a cofounder of and holds stocks in ToolGen, Inc., a company focused on therapeutic genome editing. All the other authors declare no competing financial interests.
Integrated supplementary information
(a) Sample-specific SNVs in all pairwise combinations F03, F05, and FVB (WT) (A vs B means variants present in B and not in A). All three aligned sequencing data representing WT (SRR5450998), F03 (SRR5450997), and F05 (SRR5450996) were retrieved and analyzed using Strelka and Mutect. All SNVs were filtered using dbSNP 143 and mouse genome project v5. (b,c) Venn diagrams showing homozygous (blue) and heterozygous (red) SNVs (b) and indels (c) found in the wild-type control mouse (FVB) and the two gene-edited mice (F03 and F05). Variants were identified with Platypus (v. 1.0.8) with multi-sample callingmode with default parameters; variants with coverage lower than 15 in any of the samples, and multi-allelic variants are not considered.
About this article
Cite this article
Kim, ST., Park, J., Kim, D. et al. Response to “Unexpected mutations after CRISPR–Cas9 editing in vivo”. Nat Methods 15, 239–240 (2018). https://doi.org/10.1038/nmeth.4554
Communications Biology (2020)
No excessive mutations in transcription activator-like effector nuclease-mediated α-1,3-galactosyltransferase knockout Yucatan miniature pigs
Asian-Australasian Journal of Animal Sciences (2020)
Molecular Therapy (2020)
Trends in Genetics (2020)
Genome-Wide Analysis of Off-Target CRISPR/Cas9 Activity in Single-Cell-Derived Human Hematopoietic Stem and Progenitor Cell Clones