Skip to main content

Thank you for visiting You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Response to “Unexpected mutations after CRISPR–Cas9 editing in vivo

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.


  1. 1

    Schaefer, K.A. et al. Nat. Methods 14, 547–548 (2017).

    CAS  Article  Google Scholar 

  2. 2

    Saunders, C.T. et al. Bioinformatics 28, 1811–1817 (2012).

    CAS  Article  Google Scholar 

  3. 3

    Cibulskis, K. et al. Nat. Biotechnol. 31, 213–219 (2013).

    CAS  Article  Google Scholar 

  4. 4

    Rimmer, A. et al. Nat. Genet. 46, 912–918 (2014).

    CAS  Article  Google Scholar 

  5. 5

    Kim, D. et al. Nat. Methods 12, 237–243 (2015).

    CAS  Article  Google Scholar 

  6. 6

    Cho, S.W., Kim, S., Kim, J.M. & Kim, J.-S. Nat. Biotechnol. 31, 230–232 (2013).

    CAS  Article  Google Scholar 

  7. 7

    Cho, S.W. et al. Genome Res. 24, 132–141 (2014).

    CAS  Article  Google Scholar 

  8. 8

    Smith, C. et al. Cell Stem Cell 15, 12–13 (2014).

    CAS  Article  Google Scholar 

  9. 9

    Veres, A. et al. Cell Stem Cell 15, 27–30 (2014).

    CAS  Article  Google Scholar 

  10. 10

    Suzuki, K. et al. Cell Stem Cell 15, 31–36 (2014).

    CAS  Article  Google Scholar 

  11. 11

    Iyer, V. et al. Nat. Methods 12, 479 (2015).

    CAS  Article  Google Scholar 

  12. 12

    Tsai, S.Q. et al. Nat. Biotechnol. 33, 187–197 (2015).

    CAS  Article  Google Scholar 

  13. 13

    Frock, R.L. et al. Nat. Biotechnol. 33, 179–186 (2015).

    CAS  Article  Google Scholar 

Download references


J.-S.K. is supported by a grant from the Institute for Basic Science (IBS-R021-D1).

Author information



Corresponding authors

Correspondence to Matthias Schlesner or Jin-Soo Kim.

Ethics declarations

Competing interests

J.-S.K. is a cofounder of and holds stocks in ToolGen, Inc., a company focused on therapeutic genome editing. All the other authors declare no competing financial interests.

Integrated supplementary information

Supplementary Figure 1

(a) Sample-specific SNVs in all pairwise combinations F03, F05, and FVB (WT) (A vs B means variants present in B and not in A). All three aligned sequencing data representing WT (SRR5450998), F03 (SRR5450997), and F05 (SRR5450996) were retrieved and analyzed using Strelka and Mutect. All SNVs were filtered using dbSNP 143 and mouse genome project v5. (b,c) Venn diagrams showing homozygous (blue) and heterozygous (red) SNVs (b) and indels (c) found in the wild-type control mouse (FVB) and the two gene-edited mice (F03 and F05). Variants were identified with Platypus (v. 1.0.8) with multi-sample callingmode with default parameters; variants with coverage lower than 15 in any of the samples, and multi-allelic variants are not considered.

Supplementary information

Supplementary Text and Figures

Supplementary Figure 1 and Supplementary Notes 1–2 (PDF 165 kb)

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Kim, ST., Park, J., Kim, D. et al. Response to “Unexpected mutations after CRISPR–Cas9 editing in vivo”. Nat Methods 15, 239–240 (2018).

Download citation

Further reading


Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing