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Mass spectrometry imaging goes three dimensional

Two complementary approaches improve 3D mass spectrometry imaging of biomolecules on 3D surfaces and within tissue sections and single cells.

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Figure 1: The functional principles of MALDI MSI with laser triangulation and 3D OrbiSIMS.

Kim Caesar/Springer Nature


  1. 1

    Alberts, B. et al. Molecular Biology of the Cell 6th edn. (Garland Science, 2015).

    Google Scholar 

  2. 2

    Kompauer, M., Heiles, S. & Spengler, B. Nat. Methods 14, 1156–1158 (2017).

    CAS  Article  Google Scholar 

  3. 3

    Passarelli, M.K. et al. Nat. Methods 14, 1175–1183 (2017).

    CAS  Article  Google Scholar 

  4. 4

    Norris, J.L. & Caprioli, R.M. Chem. Rev. 113, 2309–2342 (2013).

    CAS  Article  Google Scholar 

  5. 5

    Soltwisch, J. et al. Science 348, 211–215 (2015).

    CAS  Article  Google Scholar 

  6. 6

    Cole, L. (Ed.) Imaging Mass Spectrometry: Methods and Protocols (Springer, 2017).

    Book  Google Scholar 

  7. 7

    Kompauer, M. Nat. Methods 14, 90–96 (2017).

    CAS  Article  Google Scholar 

  8. 8

    Zavalin, A., Yang, J., Hayden, K., Vestal, M. & Caprioli, R.M. Anal. Bioanal. Chem. 407, 2337–2342 (2015).

    CAS  Article  Google Scholar 

  9. 9

    Bartels, B. et al. RSC Adv. 7, 9045–9050 (2017).

    CAS  Article  Google Scholar 

  10. 10

    Fletcher, J.S. & Vickerman, J.C. Anal. Bioanal. Chem. 396, 85–104 (2010).

    CAS  Article  Google Scholar 

  11. 11

    Zamboni, N., Saghatelian, A. & Patti, G.J. Mol. Cell 58, 699–706 (2015).

    CAS  Article  Google Scholar 

  12. 12

    Oetjen, J. et al. Gigascience 4, 1–8 (2015).

    Article  Google Scholar 

  13. 13

    Rzagalinski, I. & Volmer, D.A. Biochim. Biophys. Acta 1865, 726–739 (2017).

    CAS  Article  Google Scholar 

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Correspondence to Klaus Dreisewerd.

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Dreisewerd, K., Yew, J. Mass spectrometry imaging goes three dimensional. Nat Methods 14, 1139–1140 (2017).

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