Supplementary Figure 3: Raw images of 20 rounds of fluorescent switching in channel 647. | Nature Methods

Supplementary Figure 3: Raw images of 20 rounds of fluorescent switching in channel 647.

From: Profiling the transcriptome with RNA SPOTs

Supplementary Figure 3

Bright dots are the real targets while dim dots are due to nonspecific binding. The switching between each round of hybridization is complete, with minimal retention of fluorescent signals from previous round. (Scale bars: 2μm.)

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