Supplementary Figure 9: mRNA can be immobilized by polyacrylamide hydrogel on a bind-silane treated coverslips. | Nature Methods

Supplementary Figure 9: mRNA can be immobilized by polyacrylamide hydrogel on a bind-silane treated coverslips.

From: Profiling the transcriptome with RNA SPOTs

Supplementary Figure 9

(a) mRNA is trapped in the hydrogel mesh once acrylamide and bis-acrylamide monomers crosslink completely on the coverslip. (b) smFISH detection of ACTB once the total RNA is captured on a coverslip through LNA poly(d)T capturing (left) or polyacrylamide hydrogel (right). Negative control (channel 488) shows that the fluorescent signals are not coming from nonspecific sources. (Scale bars: 5μm.)

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