Supplementary Figure 8: smFISH measurement in single cells correlates with RNA SPOTs measurement in NIH/3T3 cells. | Nature Methods

Supplementary Figure 8: smFISH measurement in single cells correlates with RNA SPOTs measurement in NIH/3T3 cells.

From: Profiling the transcriptome with RNA SPOTs

Supplementary Figure 8

(a) Raw images of the 7 genes measured by smFISH in NIH/3T3 cells. (Scale bars: 5μm.) (b) The averaged RNA smFISH counts agrees with RNA SPOTs SPM (spots per million) with a Pearson correlation coefficient of 0.88, indicating RNA SPOTs quantitation is accurate. Error bars represents the standard error of the mean (SEM) across different single cells.

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