Supplementary Figure 11: Different modes of acquisition sequences. | Nature Methods

Supplementary Figure 11: Different modes of acquisition sequences.

From: Localization-based super-resolution imaging meets high-content screening

Supplementary Figure 11

4 different concentrations of Ab crosslinker (Polyclonal Ab anti-SEP) were tested (0, 1/100, 1/300, 1/1000) on HeLa cells expressing SEP::GluA1::mEos2.1, and 3 types of acquisition sequences were performed: I/ Sequential acquisition: antibodies were incubated well by well, requiring manual intervention between each well. II/ Serial acquisition: all antibody concentrations were loaded at the same time at the beginning of the acquisition (no user intervention during acquisition). The acquisition order was the well without Ab first, then the wells with 1/100, 1/300 and 1/1000 of Ab concentration. III/ Random acquisition: same as II/ except that the positions were randomly acquired between all wells.

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