Supplementary Figure 7: 2 color dSTORM acquisition. | Nature Methods

Supplementary Figure 7: 2 color dSTORM acquisition.

From: Localization-based super-resolution imaging meets high-content screening

Supplementary Figure 7

Microtubules labelled with Alexa 532 and Vimentin labelled with Alexa 647 in Cos-7 cells using the same dSTORM buffer (200mM bMEA at pH7.2) selected from the characterization of isolated fluorophores (same as Figure 2c). Super-resolution (SR) reconstructions were compared to high resolution (HR) images. Focus position was set at the bottom of the cell (approx. 300 nm above the coverslip surface in TIRF illumination at 50fps).

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