Supplementary Figure 4 : Experiments to optimize activities of MST crRNAs with dLbCpf1-VPR.

From: Inducible and multiplex gene regulation using CRISPR–Cpf1-based transcription factors

Supplementary Figure 4

Results of experiments designed to test the transfection of various amounts and ratios of expression plasmids encoding dLbCpf1-VPR and crRNAs targeted to the promoters of three endogenous human genes (HBB, AR, and NPY1R) in HEK293 cells. crRNAs were encoded as a single crRNA in one transcript (Single crRNA) or as multiple crRNAs in one transcript (MST crRNA)). Transfected cells were harvested and assayed for gene expression by RT-qPCR at three different time points post-transfection (48 hours, 72 hours, and 96 hours). Representative data shown are of three biological independent replicates with error bars representing standard deviation (SD) of three technical replicates. n.s., not significantly different as determined by Student t-test (p > 0.05); *, significantly different as determined by Student t-test (two-tailed test assuming equal variance, p< 0.05)