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Tracking microscopy enables whole-brain imaging in freely moving zebrafish

Efficient tracking and optical whole-brain imaging at single-cell resolution in freely behaving zebrafish larvae pave the way for quantitative investigation of circuits underlying complex behaviors.

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Figure 1: Imaging whole-brain neuronal activity at single-cell resolution is now possible in freely moving zebrafish larvae performing complex sensory–motor tasks.

Kim Caesar/Springer Nature

References

  1. Symvoulidis, P. et al. Nat. Methods 14, 1079–1082 (2017).

    Article  CAS  Google Scholar 

  2. Kim, D.H. et al. Nat. Methods 14, 1107–1114 (2017).

    Article  CAS  Google Scholar 

  3. Cong, L. et al. eLife http://dx.doi.org/10.7554/eLife.28158 (2017).

  4. Nguyen, J.P. et al. Proc. Natl. Acad. Sci. USA 113, E1074–E1081 (2016).

    Article  CAS  Google Scholar 

  5. Ahrens, M.B., Orger, M.B., Robson, D.N., Li, J.M. & Keller, P.J. Nat. Methods 10, 413–420 (2013).

    Article  CAS  Google Scholar 

  6. Wolf, S. et al. Nat. Methods 12, 379–380 (2015).

    Article  CAS  Google Scholar 

  7. Knafo, S. et al. eLife 6, e25260 (2017).

    Article  Google Scholar 

  8. Lim, D., Ford, T.N., Chu, K.K. & Mertz, J. J. Biomed. Opt. 16, 016014 (2011).

    Article  Google Scholar 

  9. Broxton, M. et al. Opt. Express 21, 25418–25439 (2013).

    Article  Google Scholar 

  10. Prevedel, R. et al. Nat. Methods 11, 727–730 (2014).

    Article  CAS  Google Scholar 

  11. Horstmeyer, R., Ruan, H. & Yang, C. Nat. Photonics 9, 563–571 (2015).

    Article  CAS  Google Scholar 

Download references

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Correspondence to Claire Wyart.

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Thouvenin, O., Wyart, C. Tracking microscopy enables whole-brain imaging in freely moving zebrafish. Nat Methods 14, 1041–1042 (2017). https://doi.org/10.1038/nmeth.4474

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