Supplementary Figure 5: Synchronization of hardware components. | Nature Methods

Supplementary Figure 5: Synchronization of hardware components.

From: NeuBtracker—imaging neurobehavioral dynamics in freely behaving fish

Supplementary Figure 5

(a) In addition to the timestamps obtained by the computer clock, we have recorded signals directly from the components of the system using different types of output (galvanometric mirrors: analog voltage, fluorescence camera: TTL pulses, 1x IR camera: PLC logic requiring additional input voltage). (b) Example raw signals from a 50 μs time window during which the 1x IR camera acquired at 200 Hz and magnified fluorescence images were obtained at 50 Hz (the beginning of the respective exposure times correspond to the high values in the red/green lines respectively). The blue and cyan signals are the control voltages to the galvanometric mirrors. (c) Image of the resolution target acquired for illustration by the 1x camera. (d) Example signals from the galvanometric mirrors from a raster scan of the resolution target shown in (c) resulting in 12 consecutive images shown as a montage in (e).

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