Supplementary Figure 4: Image acquisition automation and data processing pipeline. | Nature Methods

Supplementary Figure 4: Image acquisition automation and data processing pipeline.

From: NeuBtracker—imaging neurobehavioral dynamics in freely behaving fish

Supplementary Figure 4

Image acquisition automation and data processing pipeline (a) Simplified schematic showing the main connections to and from the console running the control software. (b) Screenshot acquired during one of the acquisitions, showing the GUI of the control software displaying both of the imaging channels (left: 1x behavioral tracking, right: magnified real-time view of the fluorescent imaging). (c) Flowchart of the data acquisition and processing pipeline with (d) examples from the feature matching of the SURF registration. Scalebars represents 1 mm.

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