Supplementary Figure 3: Calibration of NeuBtracker components. | Nature Methods

Supplementary Figure 3: Calibration of NeuBtracker components.

From: NeuBtracker—imaging neurobehavioral dynamics in freely behaving fish

Supplementary Figure 3

(a-c) A composite resolution target (a) was placed on top of an autofluorescent plastic slide and scanned on the MicroFixed configuration by translating the FOV of the 7x fluorescent channel to generate (b) a stitched image of the entire resolution target at high resolution. Individual tiles are shown in (c) (d-f) Both positive and negative resolution targets consisting of concentric rings are placed in the sample holder to ensure proper registration of the FOVs of the two cameras. (g-i) Profile of the fluorescence excitation analyzed by using an autofluorescent plastic slide. The red circle in (h) indicates the diameter of the arena. (i) Corresponding plot of the standard deviation/mean for all xy-galvo positions to quantify the homogeneity throughout the arena (indicated by the red area).

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