Supplementary Figure 7: Image quality assessment and performance of registration routine. | Nature Methods

Supplementary Figure 7: Image quality assessment and performance of registration routine.

From: NeuBtracker—imaging neurobehavioral dynamics in freely behaving fish

Supplementary Figure 7

The heat maps show several measures of the data shown in Figure 2 as a function of swimming speed: (a) the features detected by the initial quality check; frames with less than 5 features were discarded (b) the scaling factor of the similarity transformation obtained from the registration; this parameter was used to censor frames which deviated by more than 0.1 from unit scale (c) the registration error (ssd) from the final registration step of the remaining frames. (d) The histogram shows the imaging frames that survived the quality check and the subsequent selections based on registration quality.

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