(a) The numbers of significantly upregulated genes in hiPSC-derived SFTPC+ cells relative to CPMhi progenitor and SFTPC- cells in microarray data (n = 3 independent experiments for each condition, >2-fold, P < 0.05, ANOVA with Tukey’s post hoc) (GSE90590). (b) A pie chart showing GO term distribution for the 740 upregulated genes in hiPSC-derived SFTPC+ cells. (c) A list of 75 GO terms (“cellular component”, [33 terms]; “biological process”, [38 terms]; and “molecular function”, [4 terms]) found to be significantly enriched in SFTPC+ cell transcriptomes by Fisher’s exact test (P < 0.05) (GSE90590). The red bars correspond to GO terms in (b). (d) Heat maps of the gene expression annotated with the GO terms of AT2 cell function in comparison of hiPSC-derived CPMhi progenitor cells and SFTPC+ and SFTPC- cells. (e) A list of the top 25 upregulated genes in hiPSC-derived SFTPC+ cells. An immunofluorescent analysis was performed to detect expression of the genes shown in red. See also Supplementary Fig. 4 and Supplementary Table 1. B2-3 hiPSC line was used in all of the experiments.