Supplementary Figure 12: Validation of the scRNA-seq of hiPSC-derived FD-SFTPC+ cells. | Nature Methods

Supplementary Figure 12: Validation of the scRNA-seq of hiPSC-derived FD-SFTPC+ cells.

From: Long-term expansion of alveolar stem cells derived from human iPS cells in organoids

Supplementary Figure 12

(a) The data of the sequenced reads in P0, P2 and P5 FD-SFTPC+ single-cells. (b) The numbers of detected reads of RNA spike-in controls added to P0, P2 and P5 FD-SFTPC+ single-cell lysates, respectively. The detected reads (log10) increased according to the estimated copies of each spike-in control mixed into each lysate (shown in the text box). Error bars indicate the mean ± 2 s.d. (c) Correlation between the mean expression of each of 24,104 genes in single cells and 200 bulk cells in P0, P2 and P5 FD-SFTPC+ cells, respectively. r indicates Pearson’s correlation coefficient. (d) Correlation among the mean expression of each of 24,104 genes in P0, P2 and P5 FD-SFTPC+ single cells. r indicates Pearson’s correlation coefficient.

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