Supplementary Figure 8: Global gene expression pattern of expanded hiPSC-derived FD-SFTPC+ cells. | Nature Methods

Supplementary Figure 8: Global gene expression pattern of expanded hiPSC-derived FD-SFTPC+ cells.

From: Long-term expansion of alveolar stem cells derived from human iPS cells in organoids

Supplementary Figure 8

(a) A non-biased PCA of the transcriptomes of P0, P2 and P5 hiPSC-derived FD-SFTPC+ cells, CPMhi progenitor cells, hiPSC-derived proximal airway epithelial cells (PAECs) (n = 3 independent experiments, each) and primary isolated AT2 cells (n = 3 donors) (GSE90591). See also Figure 4f. (b) Non-biased hierarchical clustering of the transcriptomes of P0, P2 and P5 hiPSC-derived FD-SFTPC+ cells, CPMhi progenitor cells, PAECs (n = 3 independent experiments, each) and primary isolated AT2 cells (n = 3 donors). (c) Hierarchical clustering of each transcriptome (columns) based on representative lung epithelial cell markers described in Supplementary Table 3 (rows). B2-3 hiPSC line was used in all experiments, except for parental 201B7 hiPSC-derived PAECs.

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