Supplementary Figure 5: scRNA-seq analysis of differentiation from CPMhi progenitor cells to SFTPC+ cells. | Nature Methods

Supplementary Figure 5: scRNA-seq analysis of differentiation from CPMhi progenitor cells to SFTPC+ cells.

From: Long-term expansion of alveolar stem cells derived from human iPS cells in organoids

Supplementary Figure 5

(a) The data of the sequenced reads in CPMhi cells on day 14 and 21 and EpCAM+ cells on day 26. (b) The numbers of detected reads of RNA spike-in controls added to single-cell lysates of CPMhi cells on day 14 and 21, and EpCAM+ cells, respectively. The detected reads (log10) increased according to the number of estimated copies of spike-in controls mixed in each lysate (shown in the text box). Error bars indicate the mean ± 2 s.d. (c) The correlation between the mean expression of each of 24,104 genes in single cells and 200 bulk cells in CPMhi cells on day 14 and 21 and EpCAM+ cells, respectively. r indicates Pearson’s correlation coefficient. (d) The correlation among the mean expression of each of 24,104 genes in CPMhi cells on day 14 and 21 and EpCAM+ single cells. r indicates Pearson’s correlation coefficient. (e) PCA of the gene expression profiles of 206 single cells on 17,873 genes. Three-dimensional image is shown in Figure 2c (GSE90813).

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