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Acknowledgements

We thank M. Muraro and A. Lyubimova for experimental advice. We also thank the Hubrecht FACS facility, the Hubrecht Single Cell facility, the Hubrecht Imaging facility and the Utrecht Sequencing Facility (USF). This work was supported by a Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO) TOP award (NWO-CW 714.016.001) to A.v.O. and by an European Research Council grant (ERC 220-H75001EU/HSCOrigin-309361) and the UMC Utrecht “Regenerative Medicine & Stem Cells” priority research program to C.R. Pax7nGFP mice were kindly provided by S. Tajbakhsh (Institut Pasteur, Paris, France).

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Author notes

    • Susanne C van den Brink
    •  & Fanny Sage

    These authors contributed equally to this work.

Affiliations

  1. Hubrecht Institute–KNAW and University Medical Center Utrecht, Utrecht, the Netherlands.

    • Susanne C van den Brink
    • , Fanny Sage
    • , Ábel Vértesy
    • , Bastiaan Spanjaard
    • , Josi Peterson-Maduro
    • , Chloé S Baron
    • , Catherine Robin
    •  & Alexander van Oudenaarden
  2. University Medical Center Utrecht, Cancer Genomics Netherlands, Utrecht, The Netherlands.

    • Susanne C van den Brink
    • , Ábel Vértesy
    • , Bastiaan Spanjaard
    • , Josi Peterson-Maduro
    • , Chloé S Baron
    •  & Alexander van Oudenaarden
  3. Berlin Institute for Medical Systems Biology, Max Delbrück Center for Molecular Medicine, Berlin-Buch, Germany.

    • Bastiaan Spanjaard
  4. University Medical Center Utrecht, Department of Cell Biology, Utrecht, The Netherlands.

    • Catherine Robin

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Competing interests

The authors declare no competing financial interests.

Corresponding author

Correspondence to Alexander van Oudenaarden.

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https://doi.org/10.1038/nmeth.4437