Recent reports on the characteristics of naive human pluripotent stem cells (hPSCs) obtained using independent methods differ. Naive hPSCs have been mainly derived by conversion from primed hPSCs or by direct derivation from human embryos rather than by somatic cell reprogramming. To provide an unbiased molecular and functional reference, we derived genetically matched naive hPSCs by direct reprogramming of fibroblasts and by primed-to-naive conversion using different naive conditions (NHSM, RSeT, 5iLAF and t2iLGöY). Our results show that hPSCs obtained in these different conditions display a spectrum of naive characteristics. Furthermore, our characterization identifies KLF4 as sufficient for conversion of primed hPSCs into naive t2iLGöY hPSCs, underscoring the role that reprogramming factors can play for the derivation of bona fide naive hPSCs.
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We thank the high-quality cell sorting service and technical input provided by Monash Flowcore Facility and J. Hatwell-Humble for conducting the teratomas experiments. We also thank the Cytogenetics department of Monash Pathology for help with G-banding karyotype analysis. Furthermore, the authors thank the ACRF Centre for Cancer Genomic Medicine at the MHTP Medical Genomics Facility for assistance with next-generation library preparation and Illumina sequencing. This work was supported by National Health and Medical Research Council (NHMRC) project grants APP1104560 to J.M.P. and A.L.L., APP 1069830 to R.L. and a Monash University strategic grant awarded to C.M.N. X.L. was supported by a Monash International Postgraduate Research Scholarship and a Monash Graduate Scholarship. A.S.K. was supported by an NHMRC Early Career Fellowship APP1092280. J.M.P. and R.L. were supported by Silvia and Charles Viertel Senior Medical Research Fellowships.
J.M.P. is a director in Cell Mogrify; however, the work presented in this manuscript is not related to Cell Mogrify.
Integrated supplementary information
(a) Experimental outline for the media conversion approach to toggle primed hiPSCs to a naive state (note: naive t2iLGöY culture condition was insufficient for conversion). (b) Resulting colony morphologies (Scale bar, 250μm) and (c) Immunofluorescence labelling for classical pluripotency markers TRA-1-60, SSEA3, NANOG, OCT4 and naive pluripotency associated markers KLF17 and TFE3 (Scale bar, 25μm).
(a) viSNE map (related to Fig. 1d) coloured for expression levels of cell surface markers CD24, SSEA3, SSEA4, F11R, NLGN4X, TRA-1-60. (b) Histogram of flow cytometry data for detection levels of cell surface marker F11R in primed and naive rt2iLGöY hiPSCs (representative histogram derived from n=3 cell donors 32F, 38F and 55F). (c) MDS plot of primed and different naive hiPSCs derived in this study. Two donors (32F and 55F) were used to generate all the hiPSCs except for cNHSM (one donor, 32F). Each dot represents a different cell culture sample per indicated condition. (d) Growth curves of primed and naive r5iLAF and rt2iLGöY iPSCs (n=3 culture replicates from 32F, error bars, s.e.m.). (e) Bright field image (Scale bar, 250μm) and immunofluorescence staining (Scale bar, 25μm) of primed iPSCs obtained by differentiation of naive rt2iLGöY hiPSCs, (32F).
Supplementary Figure 3 Generation of primed and naive rt2iLGöY hiPSCs via an mRNA reprogramming system.
(a) Schematic representation of experimental design for somatic cell reprogramming via mRNA transfections of human fibroblasts into primed and t2iLGöY hiPSCs. (b) Bright field images (Scale bar, 100μm) and immunofluorescence staining (Scale bar, 25μm) of primed and established rt2iLGöY hiPSCs. (c) Multidimensional representation of flow cytometry data for primed and t2iLGöY hiPSCs reprogrammed by mRNA approach by viSNE map (left) and SPADE tree (right). (d) MDS of primed (one donor, 32F) and t2iLGöY (one donor 32F) hiPSCs reprogrammed by mRNA approach as well as primed (two donors, 32F and 55F) and naive rt2iLGöY hiPSCs (two donors, 32F and 55F) reprogrammed by Sendai approach integrated with a previously published human ICM dataset21. Each dot represents a different cell culture sample per indicated condition.
Supplementary Figure 4 Additional characterisation of naive ct2iLGöY hPSCs generated by overexpression of OSKM or KLF4 alone.
(a) Immunofluorescence labelling of established primed hiPSCs, hESCs, naive ct2iLGöY K hiPSCs and ct2iLGöY K hESCs, Scale bar, 25μm. (b) Table with karyotypes of naive ct2iLGöY K hESCs/hiPSCs at indicated passages. (c) Dome-shaped colony counts of primed hiPSCs transduced with OSKM or KLF4, two passages post transduction (n=3 ± s.e.m., **, p=0.0087). (d) FACS analysis of primed hiPSCs transduced with OSKM or KLF4 at p0, p1, p2, percentages of F11R++/SSEA3/EPCAM+ cells of live cells were recorded (n=3 ± s.e.m., *, p=0.0469 **, p=0.0023). (e) Representative FACS blots of gating strategies used for the analysis depicted in panel d.
Supplementary Figure 5 Additional characterisation of naive ct2iLGöY hPSCs generated by overexpression of OSKM or KLF4 alone.
(a) MDS plot of all datasets (samples generated in this study plus published datasets3-5, 21). Two donors (32F and 55F) were used to generate all the iPSCs except for cNHSM (one donor, 32F), for mRNA reprogramming, primed (one donor, 32F) and t2iLGöY (one donor, 32F) and for primed to t2iLGöY conversion (three donors, H9 ESCs, 32F iPSCs and 38F iPSCs for both ct2iLGöY OSKM and ct2iLGöY K conversion). Each dot represents a different cell culture sample per indicated condition. (b) PCA of single cell data for primed hiPSCs (32 cells), naive rt2iLGöY hiPSCs (41 cells), naive ct2iLGöY OSKM hiPSCs (19 cells) and naive ct2iLGöY K hiPSCs (18 cells); 32F. (c) Schematic representation of experimental design for conversion of primed hPSCs to naive ct2iLGöY hPSCs by transfections with KLF4 mRNAs. (d) Bright field images (Scale bar, 100μm) and immunofluorescence labelling (Scale bar, 25μm) of human naive ct2iLGöY hiPSCs/hESCs generated by mRNA transfections of primed hiPSCs/hESCs using KLF4 mRNAs in t2iLGöY condition.
(a) Representative FACS blots of analysis of primed cells transduced with or without KLF4 (6 days post transduction) in t2iLGöY medium. (b,c) Combined gene ontology analysis for DEGs (FDR<0.05, LogFC>1) between day 2 samples (with/without KLF4) and for DEGs between day 6 samples (with/without KLF4 in t2iLGöY medium). The contribution of the day 2 and day 6 datasets is indicated in red and blue respectively in panel b and gene ontology categories including FDR are indicated in panel c. (two donors, 32F and 38F) were analysed by RNA sequencing per condition).
Supplementary Figures 1–7 and Supplementary Tables 1, 2, 4–7.
Establishment and maintenance of human naive pluripotent stem cells by primed to naive conversion and reprogramming of fibroblasts.
Proteins detected in primed and naive rt2iLGöY iPSCs by mass spectometry.
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Liu, X., Nefzger, C., Rossello, F. et al. Comprehensive characterization of distinct states of human naive pluripotency generated by reprogramming. Nat Methods 14, 1055–1062 (2017). https://doi.org/10.1038/nmeth.4436
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