Supplementary Figure 6: Split-CPTS2.0-mediated optogenetic gene activation with multiple sgRNAs. | Nature Methods

Supplementary Figure 6: Split-CPTS2.0-mediated optogenetic gene activation with multiple sgRNAs.

From: CRISPR–Cas9-based photoactivatable transcription systems to induce neuronal differentiation

Supplementary Figure 6

Light-induced upregulation of MYOD1 (a) and IL1RN (b) in HEK293T cells using Split-CPTS2.0 and CPTS with a single sgRNA and quadruple sgRNAs targeting MYOD1 and IL1RN, respectively. Data are expressed as relative mRNA amount to the negative control transfected with empty vector in the dark and represented as mean ± s.e.m. (n=6 from three individual experiments with two cell culture replicates). D, Dark; L, Light. Welch’s two-tailed t test was performed. *p < 0.05, ** < 0.01, ***p < 0.005 versus the sample in the dark.

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