Light-induced ASCL1 upregulation in HeLa cells (a) and human fetal fibroblasts (b) using Split-CPTS2.0, CPTS, LACE, dCas9-VP64 and SAM with a single sgRNA. Data are expressed as relative ASCL1 mRNA amount to the negative control transfected with empty vector or EGFP in the dark and represented as mean ± s.e.m. ((a) n=4 from two individual experiments with two cell culture replicates. (b) n=2 from two individual experiments). D, Dark; L, Light.