Supplementary Figure 14: Split-CPTS2.0-mediated neuronal differentiation of human iPSCs (201B7). | Nature Methods

Supplementary Figure 14: Split-CPTS2.0-mediated neuronal differentiation of human iPSCs (201B7).

From: CRISPR–Cas9-based photoactivatable transcription systems to induce neuronal differentiation

Supplementary Figure 14

(a) Immunofluorescence images of 201B7-derived neurons induced by split-CPTS2.0 targeting NEUROD1. Cells were immunostained after 4 days blue light stimulation or dark incubation. (b) Immunofluorescence images of 201B7 cells expressing indicated dCas9-based photoactivatable transcription systems and sgRNAs. Cells were immunostained after 4 days blue light stimulation or dark incubation. Magenta, beta III tubulin; Blue, DAPI; Green, EGFP. Scale bar = 50 μm.

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