Supplementary Figure 10: DNA-targeting specificity analysis of Split-CPTS2.0 and CPTS2.0. | Nature Methods

Supplementary Figure 10: DNA-targeting specificity analysis of Split-CPTS2.0 and CPTS2.0.

From: CRISPR–Cas9-based photoactivatable transcription systems to induce neuronal differentiation

Supplementary Figure 10

(a) Luciferase reporter is constructed of Cas9-binding sites, minimal CMV promoter and luciferase gene. (b) DNA targeting specificity analysis of Split-CPTS2.0, CPTS2.0, dCas9-VP64 and SAM using luciferase reporter plasmid activation assay. The positions of mutations in each sgRNA are indicated in red. Values are normalized to positive control with perfectly matched sgRNAs. Data are shown as the mean ± s.e.m. (n=6 from two individual experiments with three cell culture replicates).

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